Chromatography of plant pigments

Separation of Plant Pigments Using Chromatography Paper chromatography is a useful technique in the separation and identification of different plant pigments. In this technique, the mixture containing the pigments to be separated is first applied as a spot or a line to the paper about 1.

Chromatography of plant pigments

How to Write a Summary of an Article? The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent. The solvent carried the dissolved pigments as it moved up the paper.

The pigments were carried at different rates because they were not equally soluble. The most soluble pigment traveled the longest distance while the others traveled in a shorter length.

Objectives

The distance of the pigment traveled was unique for that pigment in set conditions and was used to identify the pigment. The ratio was then used to measure the Rf retention factor value.

Chromatography of plant pigments

Glucose is a simple carbohydrate that provides immediate fuel to cells but it is also a building block for more complex carbohydrates stored by living organisms for future use. For photosynthesis to transform light energy from the sun into chemical energy bond energy in plants, the pigment molecules absorb light to power the chemical reactions.

Plant pigments are macromolecules produced by the plant, and these pigments absorb specified wavelengths of visible light to provide the energy required for photosynthesis.

Chlorophyll is necessary for photosynthesis, but accessory pigments collect and transfer energy to chlorophyll. Although pigments absorb light, the wavelengths of light that are not absorbed by the plant pigments are reflected back to the eye. The reflected wavelengths are the colors we see in observing the plant.

Plants contain different pigments, and some of the pigments observed include: Flavonoids, anthocyanins, and carotenoids are just some of the categories of plant pigments known to have antioxidant properties.

Many lines of research suggest that consuming a diet rich in plant pigments may slow the process of cellular aging and reduce the risks of some types of disease, such as cancer, heart disease, and stroke.

The point of this experiment is to look at the polarity of some of the common pigments in plant leaves and how that polarity affects their interactions with the cellulose fibers in paper and a few solvents and to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends.

These solvents are used because they are capable of separating mixtures that contain both polar and non-polar compounds, or to increase separation of mixtures of compounds that have similar behavior with a single solvent.

As the solvent slowly travels up the paper, the different components of the extract travel at different rates and the extracts are separated into different colors. After minutes, the distance traveled by each pigment and solvent were measured. Pigments and Rf values for each plant extract The distance traveled relative to the solvent is called the Rf value, or the Retardation value.

It can be computed with the formula: It also means that it is less polar because it interacts less strongly with the polar absorbent on the filter paper.

Introduction

So similarly, the smaller the Rf value a compound has, the shorter the distance it travelled. It also means that is is more polar because it interacts more strongly with the polar absorbent on the filter paper.

Chromatography of plant pigments

The distances travelled by the pigments were significantly different than ours because they used different percentages of solvents. The pigments dissolved in the solvent and migrated upward. We have always understood chlorophyll, a pigment that is very important in photosynthesis, to be green.

However, through this experiment we have discovered that many other pigments are also present in the leaves. For example the kangkong leaf also contains different pigments even though the leaf is dominated by the color green.

Foundations of Organic Chemistry. Cengage Learning Asia Pte Ltd. Illustrated guide to Home Chemistry experiments.Chromatography of leaves. Class practical. Students use chromatography to separate the pigments in a leaf.

Lesson organisation. This experiment takes about 30 minutes and can be conveniently carried out in groups of 2 or 3 . Chromatography of Plant Pigments INTRODUCTION: Chlorophyll often hides the other pigments present in leaves.

In Autumn, chlorophyll breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to show their colors. The mix of pigments in a leaf may be separated into bands of color by the technique of paper chromatography.

Chromatography is used to study plant pigment by extracting pigments from the plant and then sorting them by their physical characteristics so they can be analyzed. Chromatography is useful in separating different types of plant pigments because they are very similar to one another and can be. Chromatography of Plant Pigments INTRODUCTION: Chlorophyll often hides the other pigments present in leaves.

In Autumn, chlorophyll breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to show their colors. The mix of pigments in a leaf may be separated into bands of color by the technique of paper chromatography.

Thin Layer Chromatography with Plant Pigments Thin layer chromatography is an important analytical test for identifying unknown compounds, monitoring reactions, and testing chemical purity.

The purpose of this experiment was to acquire the TLC technique. Plant Traveling Lab. TTU/HHMI at CISER. 2 The bands derived in paper chromatography contain the pigments found in the plant. The bands can be cut apart, and .

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